Fig. 1

BRG1 is increased in PD mice and in TGF-β1-induced or PD fluid-treated peritoneal mesothelial cells. a BRG1 was detected by immunohistochemical staining. Representative micrographs show the expression and localization of BRG1 in parietal peritoneum from PD mice. Scale bar, 50 μm. Saline, saline-injected control group. PDF, PD fluid containing 4.25% glucose-injected group. b Western blot analyses of visceral peritoneum expression of BRG1, Fibronectin, a-SMA in different groups are presented. Numbers (1, 2 and 3) represent different samples in a given group. c HMrSV5 cells were treated with TGF-β1 (5 ng/ml) for the indicated time period. Western blot analyses of expression of BRG1, Fibronectin, a-SMA in different groups are presented. d HMrSV5 cells were exposed to the mixture of 4.25%PD fluid(PDF) and culture medium by 1:4,1:3 and 1:2,respectively. Cells incubated in medium diluted 1:2 with sterile saline served as a control. Western blot analyses of expression of BRG1, Collagen I, TGF-β1, E-cadherin in different groups are presented. Numbers (1, 2 and 3) represent different samples in a given group. e Primarily cultured rat peritoneal mesothelial cells(RPMCs) exhibited cobble-like appearance under light microscope. Scale bar, 100 μm. f Top: cellular immunofuorescence staining revealed that E-cadherin (red) was positive in primarily cultured RPMCs. Bottom: cellular immunofuorescence staining revealed that Vimentin(green) was positive but a-SMA (red) was nagetive in primarily cultured RPMCs.Scale bar, 50 μm. g RPMCs were treated without or with TGF-β1 (5 ng/ml) for 48 h. Western blot analyses of expression of BRG1, Collagen I, Fibronectin, E-cadherin, Vimentin in different groups are presented. Numbers (1, 2 and 3) represent different samples in a given group. h RPMCs were exposed to the mixture of 4.25%PDF and culture medium by 1:2. Cells incubated in medium diluted 1:2 with sterile saline served as a control. Western blot analyses of expression of BRG1, Collagen I, E-cadherin, TGF-β1, a-SMA in different groups are presented. Numbers (1, 2 and 3) represent different samples in a given group