Fig. 2

Effect of DMI and GBR12935 on mIBG internalization in HEK-NET and HEK-DAT cells. A–D mIBG internalization in HEK-NET and HEK-DAT cells treated with DMI or GBR12935. Curves were obtained through nonlinear regression and fit function of GraphPad Prism 9.1. 100% represent mIBG 10 nM, as the concentration added in the cell medium. E and F mIBG internalization in HEK-NET and HEK-DAT cells treated with or without DMI and GBR12935. Results were normalized by a BCA test. Statistical analysis was performed using the unpaired t-test between the control and condition tested. ****p < 0.0001. All the experiments were repeated at least three times in duplicates, with the mean values of each series shown for A–D