Fig. 5

Exo-si-FTO efficiently suppressed FTO expression and dopaminergic neuronal death in PD model in vitro. A Electron microscope scanning of exosomes from MSCs. B Nanoparticle tracking analysis of exosomes from MSCs. C Western blotting analysis of TSG101,CD81, Synthenin-1, HSP90B1 and Calnexin protein expressions in exosomes from MSCs. D Confocal microscopy analysis of the cellular distributions of exosomes which was stain with DID (red) in MN9D dopaminergic neurons. The cellular morphology was stained with FITC-Phalloidin (Green). E qRT-PCR analysis of FTO mRNA expression in MN9D dopaminergic neurons incubated with MSC-exo-si-NC or MSC-exo-si-FTO (n = 3). F Western blotting analysis of FTO expression in MN9D dopaminergic neurons incubated with MSC-exo-si-NC or MSC-exo-si-FTO. G Flow cytometry analysis of apoptosis of MN9D dopaminergic neurons incubated with MSC-exo-si-NC or MSC-exo-si-FTO, in the presence of MPP + (n = 3). Data are represented as mean ± SD, *represents P < 0.05, **represents P < 0.01