Fig. 4

FTO promotes ATM expression through m6A-dependent stabilizing of ATM mRNA in dopaminergic neurons. A The potential m6A sites on ATM mRNA were analyzed by m6Avar. B qRT-PCR analysis of the indicated genes in MN9D dopaminergic neurons transfected with si-NC or si-FTO (n = 3). C qRT-PCR analysis of the indicated genes in MN9D dopaminergic neurons transfected with the empty control plasmid or pCMV-Flag-FTO plasmid (n = 3). D qRT-PCR analysis of ATM mRNA in MN9D dopaminergic neurons transfected with pCMV-Flag-FTO plasmid or treated with MPP + (n = 3). E qRT-PCR analysis of ATM mRNA in MN9D dopaminergic neurons co-transfected with pCMV-Flag-FTO plasmid + pCMV-Myc vector or pCMV-Flag-FTO plasmid + pCMV-Myc-Mettl3 (n = 3). Data are represented as mean ± SD, *represents P < 0.05, **represents P < 0.01