Fig. 3

Knockdown of FTO inhibits ATM expression and alleviates dopaminergic neuronal death in PD models in vitro. A Heatmap of the differentially expressed genes in the striatum of 6 cases of PD patients and 6 cases of healthy persons from GSE54282 datasets. B The pathway and process enrichment analysis of the significantly upregulated genes through Metascape. C Heatmap of the significantly upregulated genes which are involved in neuronal death. D qRT-PCR analysis of the indicated genes in the mice PD model (n = 3). E qRT-PCR analysis of FTO mRNA expressions in MN9D dopaminergic neurons transfected with si-NC or si-FTO (n = 3). F qRT-PCR analysis of the indicated genes in MN9D dopaminergic neurons transfected with si-NC or si-FTO and treated with MPP + (n = 3). G Western blotting analysis of the indicated protein expressions in MN9D dopaminergic neurons transfected with si-NC or si-FTO and treated with MPP + . H, I and J Immunofluorescence staining analysis of ATM, TH, and α-Syn expressions in MN9D dopaminergic neurons transfected with si-NC or si-FTO and treated with MPP + (n = 3). K Flow cytometry analysis of apoptosis of MN9D dopaminergic neurons transfected with si-NC or si-FTO and treated with MPP + for 24 h (n = 3). Data are represented as mean ± SD, *represents P < 0.05, **represents P < 0.01