Fig. 1

The m6A level of dopaminergic neurons significantly reduced in PD models in vitro and in vivo. A Cell viability of mouse MN9D dopaminergic neurons treated with different concentrations of MPP + for 24 h (n = 3). B Western blotting analysis of TH and α-Syn protein expressions in the MN9D dopaminergic neurons treated with 500 μM MPP + for 24 h. C Flow cytometry analysis of apoptosis of MN9D dopaminergic neurons treated with 500 μM MPP + or not for 24 h (n = 3). D Detection of the total RNA m6A methylation levels in the MN9D dopaminergic neurons treated with 500 μM MPP + for 24 h (n = 3). E Morris water maze assay analysis of the Control and PD mice (n = 6). F IHC analysis of α-Syn in the striatum of mice PD model (n = 3). G Immunofluorescence staining analysis of dopaminergic neuronal apoptosis in the striatum of mice PD model (n = 3). H IHC analysis of m6A in the striatum of mice PD model (n = 3). Data are represented as mean ± SD, *represents P < 0.05, **represents P < 0.01