Table 1 Primer sequences for real-time qPCR analysis
Gene name | Forward primer (5′–3′) | Reverse primer (5′–3′) |
|---|---|---|
Abca4 | CAGAAGATTCGCTTTGTAGTGGA | CCTTGTTGGGAAAATGGCATTC |
Aim2 | GTCACCAGTTCCTCAGTTGTG | CACCTCCATTGTCCCTGTTTTAT |
Axl | ATGGCCGACATTGCCAGTG | CGGTAGTAATCCCCGTTGTAGA |
Bbs9 | AGCCACCAATGTGGAACCTGGA | CTGTAGTGGAGGCTGCACATAG |
Casp4 | GTGGTGAAAGAGGAGCTTACAGC | GCACCAGGAATGTGCTGTCTGA |
Casp8 | CGGTGAAGAACTGCGTTTCC | ACGCCAGTCAGGATGCTAAG |
Ccl2 | AGCTGTAGTTTTTGTCACCAAGC | GTGCTGAAGACCTTAGGGCA |
Cd68 | GGCGGTGGAATACAATGTGTCC | AGCAGGTCAAGGTGAACAGCTG |
Clec7a | GACTTCAGCACTCAAGACATCC | TTGTGTCGCCAAAATGCTAGG |
Cnga1 | CGAGCCATTTGTGCTGCTTA | TCATGGTTAGTTTAATATCTGCGCT |
Crx | CCAATGTGGACCTGATGCACCA | GTACTGGGTCTTGGCAAACAGG |
Gfap | CCGAGTACTGAAGCCAAGGG | GCAGTTTGTAACCCCTCCCA |
Glul | GAGGAGAATGGTCTGAAGTGC | ACCGGCAGAAAAGTCGTTGA |
Gnat1 | CCCGACTACGATGGACCTAAC | TTGACGTTCTGTGTGTCGGT |
Guca1b | CTGGACATTGTGGAGGCGAT | GACAGCTGGCCGTCTCCATT |
Il1b | TGCCACCTTTTGACAGTGATG | AAGGTCCACGGGAAAGACAC |
Mlkl | CTGAGGGAACTGCTGGATAGAG | CGAGGAAACTGGAGCTGCTGAT |
N2re3 | GCCTTATCACCGCCGAAACTTG | CATGGATGCCATCCAGACTGCA |
Naip2 | AGCTTGGTGTCTGTTCTCTGT | GCGGAAAGTAGCTTTGGTGTAG |
Nlrp3 | ATTACCCGCCCGAGAAAGG | TCGCAGCAAAGATCCACACAG |
Nrl | CTCTTGGCTACTATTCAGGGAGC | GGTTCAACTCGCGCACAGACAT |
Nxnl1 | GGAACAACAGCGACCAGGAT | GTGAGCCGCACGAAGAAGT |
Opn1mw | AGCCCTTTGGCAATGTGAGA | AAGGCCAGTACCTGCTCCAA |
Opn1sw | TCATCTTCTGTTTCATCATTCCTCT | CTTTTGTGTCGTAGCAGACTCTT |
P2ry12 | ATGGATATGCCTGGTGTCAACA | AGCAATGGGAAGAGAACCTGG |
Pde6b | TGGAGAACCGTAAGGACATCGC | TCCTCACAGTCAGCAGGCTCTT |
Prph2 | GCAATCGCTACCTGGACTTCTC | GTGAGCTGGTACTGGATACAGG |
Rdh12 | ATCTTGGTACTGCTTACGTCCT | CACCAGCAAAGAACTTCCTGA |
Reep6 | CGGTTACGGGGCCTCTCTA | CCAGTAGGTTAGCCACACAGT |
Rho | CCTTTGTCATCTACATGTTCGTGGT | CTTCCTTCTCTGCCTTCTGAGTGGT |
Ripk1 | GACTGTGTACCCTTACCTCCGA | CACTGCGATCATTCTCGTCCTG |
Ripk3 | CCACACCAGCAAGGACATCT | GCCGAACTTGAGGCAGTAGT |
Rom1 | TGGGTCAGCAACCGTTACTTGG | GAGAGTTGGCTTTGCAGGCAAG |
Rp1l1 | TGTGACTGCGAGGAGTGAACGT | TCAAGGAAACCTGCCGCAGCTT |
Rpgrip1 | GACCACGAAGAGAAACTGGAGC | CAGAGTGCCATACGCGACATCT |
Slc24a1 | GTCAAGGTCTGAAGGTTTGGG | TCTTTGGTCGGAGTAACCGC |
Tmem119 | CCTACTCTGTGTCACTCCCG | CACGTACTGCCGGAAGAAATC |
Tnf | ACGTCGTAGCAAACCACCAA | GCAGCCTTGTCCCTTGAAGA |
Tspo | GAGCCTACTTTGTACGTGGCGA | GCTCTTTCCAGACTATGTAGGAG |
Ush2a | TGCTCAGTGACCCTGTTTCCGT | TTGTTGCGAGCTGGTGTAGACC |
18S rRNA | GAGGTTCGAAGACGATCAGA | TCGCTCCACCAACTAAGAAC |