Fig. 5

Hyperoside protects against light-induced impairment of photoreceptor morphological integrity. A Representative HE-stained microscopic images. Scale bar, 50 μm. B The number of photoreceptor nuclei was counted at 500 μm away from ONH in the superior retina. C Representative microscopic images showing the expression pattern of rhodopsin, M-opsin and S-opsin (in red) in the retina. DAPI (in blue) was counterstained to label the nuclei. White arrowheads indicated disordered, mislocalized or residual rhodopsin expression as well as mislocalized or absence of M-opsin and S-opsin expression. Scale bar, 50 μm. Data were expressed as mean ± SEM (n = 4 per group). ***Compared to NLE, P < 0.001; ^###compared to LE, P < 0.001; ns, not significant. HYP the light-exposed mice treated with 50 mg/kg hyperoside, INL inner nuclear layer, IS inner segment, LE the light-exposed mice treated with vehicle, NLE the vehicle-treated mice without light exposure, ONL outer nuclear layer, OS outer segment