Fig. 4

Inhibiting caspase-1 prevents the OGD-induced decrease in GluA1 expression in the plasma membrane. a Western blotting was used to detect the expression of cytoplasmic AMPARs in cultured neurons following OGD. b, c The expression of GluA1 (b), but not GluA2 (c), was notably decreased in the total cell lysates of cultured neurons following OGD. d Western blotting was used to detect the expression of AMPARs in the plasma membrane of cultured neurons following OGD. e, f The expression of GluA1 (e) and GluA2 (f) were notably decreased in the plasma membrane of cultured neurons following OGD. g The expression of AMPARs in the plasma membrane of cultured neurons treated with AC-YVAD-CMK (5 µM) 1 h before OGD was detected by Western blotting. h, i The expression of GluA1 (h), but not GluA2 (i), was restored in the plasma membrane of cultured neurons after AC-YVAD-CMK treatment. The data were presented as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001