Fig. 2

The NLRP3 inflammasome signaling pathway is activated in cultured primary neurons subjected to OGD. a The expression of NLRP3 inflammasome proteins in cultured neurons after OGD was detected by Western blotting. b The expression of NLRP3 showed an inverted U-shape and peaks at 9 h following OGD. c–f The protein levels of caspase-1 (c), clv-caspase-1 (d), pre-IL-1β (e), and mature IL-1β (f) were significantly increased in neurons following OGD in comparison with the control. The data were presented as the mean ± SEM. *p < 0.05, **p < 0.01