Fig. 4

Taxonomy and developmental trajectory of malignant breast cancer cells. A BRCAs were ordered according to pseudotime trajectories and color-coded by cluster. Pie charts showing changes in malignant cell subclusters in the angiogenesis and non-angiogenesis groups. B Fish plot of the clonal lineages of major tumor subclones. C Heatmap-pathways showing genes regulated by malignant subclonal cells and signaling pathways. D Identification of regulon modules, along with representative transcription factors, corresponding binding motifs, and associated cell types. E InferCNV profiles of malignant cell subclusters. Cell-specific CNVs regulated the expression of genes involved in the evolution of angiogenic phenotypes in tumor cells. F Heatmap showing the transcriptional expression regulation of cell-specific TFs