Fig. 3

GACs with high or low CLDN6 levels express distinct biological pathways. A, B Volcano plots of dysregulated genes in GAC compared to benign tissue dichotomized by CLDN6 expression above and below the best survival-associated cutoff. A log2 fold change (log2FC) ≥ 1.5 was considered for upregulation, and a log2FC of ≤ -1.5 for downregulation. An adjusted p-value < 0.05 (Wald test, Bonferroni correction) was considered significant. C, D Exclusively upregulated and downregulated pathways in GAC with CLDN6 expression above and below the best survival-associated cutoff and the top 20 exclusively up- and downregulated genes in both subgroups. CLDN6 Claudin 6, GO BP GO Biological Process 2021, KEGG KEGG 2021 Human, GO CC GO Cellular Compartment 2021, BioPlanet BioPlanet 2019; p-values and levels of significance: *** p adjusted < 0.001, ** p adj. < 0.01, *p adj. < 0.05 (Enrichr pathway allocation with Bonferroni correction)