Fig. 2

EACs with high or low CLDN6 expression have distinct biological profiles. A, B Volcano plots of dysregulated genes in EAC compared to benign tissue dichotomized by CLDN6 expression above and below the best survival-associated cutoff. A log2 fold change (log2FC) ≥ 1.5 was considered for upregulation, and a log2FC ≤ -1.5 for downregulation. An adjusted p-value < 0.05 (Wald test, Bonferroni correction) was considered significant. C Exclusively upregulated pathways in EAC with CLDN6 expression above and below the best survival-associated cutoff and the top 20 exclusively upregulated genes in both subgroups, respectively. D Exclusively downregulated pathways and top 20 downregulated genes in CLDN6-high and -low EAC. CLDN6 Claudin 6, WP WikiPathway Human 2021, GO BP GO Biological Process 2021, KEGG KEGG 2021 Human, GO MF GO Molecular Function 2021.P. P-values and levels of significance: *** p adjusted < 0.001, ** p adj. < 0.01, *p adj. < 0.05 (Enrichr pathway allocation with Bonferroni correction)