Fig. 6

Genome-wide and site-specific validation of co-factor equilibration between AR dual-functions. A Annotation of 8q24-MYC locus with VCaP AR ChIP-Seq, H3K27ac ChIP-Seq, BRD4 ChIP-Seq, FoxA1 ChIP-Seq and HoxB13 ChIP-Seq. Blue rectangle frames: AR sites, strong FOXA1/HOXB13 binding, no H3K27ac/BRD4 increase after DHT treatment; Red rectangle frames: AR sites, week FOXA1/HOXB13 binding, H3K27ac/BRD4 increase after DHT treatment; Black rectangle frames: Week/No AR binding, strong FOXA1/HOXB13 binding, H3K27ac/BRD4 decrease after DHT treatment. B, C Global co-factor re-distribution profiling of VCaP and LNCaP ChIP-Seq datasets, aiming to display androgen effects on AR, FOXA1, and HOXB13 binding sites upon androgen treatment. Specifically, FOXA1/HOXB13 sites were divided into FOXA1/HOXB13-unique and FOXA1/HOXB13-AR common sites; and these two sets responded distinctly to androgen. See Additional file 1: Fig. S11 for additional information