Fig. 2

In PCa cells, the 8q24-MYC locus is clustered with AR binding peaks that were in annotation with enhancer hallmarks and distinct androgen responsiveness. A Annotation of 8q24-MYC locus with 22RV1 HiC, VCaP AR ChIP-Seq, VCaP H3K27ac ChIP-Seq, VCaP CTCF ChIP-Seq, clinical AR ChIP-Seq (benign, primary PCa, and CRPC) and AR motif scan. A CTCF peak right on the MYC-Promoter borders two TADs in left and right arms of this locus, respectively. P1–P14: major AR binding peaks in the 8q24-MYC locus that were highlighted and aligned from centromeric to telomeric directions. Also highlighted was the P11 site that contains multiple AR binding motifs. B VCaP cells in CDS medium were treated with 10 nM of DHT for 4 h, followed by AR ChIP-qPCR analysis of randomly selected AR binding sites (ABSs) as normalized to input. PSA enhancer (PSA-Enh) was used as positive control while NC (negative control) is an AR-irrelevant chromatin site. Data are mean values ± SD for three biologically independent samples