Fig. 1

Study design and single-cell landscape of cell types identified from myasthenic crisis (MC). a. The design of the study. This is a self-comparative study. The single-cell RNA sequencing (ScRNA-seq) was performed on peripheral blood mononuclear cells (PBMCs) from three (acetylcholine receptor antibody positive) AChR + myasthenic crisis (MC) patients. The sequencing was conducted at the most acute stage (on ventilation) and three months after the crisis. Hemogram results from 22 AChR + MC patients were also analyzed to obtain an overview of the whole blood. b. A total of 33577 PBMC cells are identified in ScRNA-seq, among which 18114 are from the acute stage of MC and 15463 from three months after MC. The dimensional reduction is performed with the uniform manifold approximation and projection (UMAP). c. The single-cell landscape at the acute stage of MC. d. The single-cell landscape after three months after the crisis. Treg regulatory T; Tcm central memory T; Tem effector memory T; Temra terminally differentiated effector memory T; DC dendritic cell; NK natural killer