Fig. 6

The effect of SNC on oxygen consumption in the spinal cord. A Protocol steps and respiratory state that were examined. The protocol examined three mitochondrial respiration states (LEAK, OXPHOS, and ET) by stimulation/ inhibition complex-I (NADH), complex-II (Succinate), and ATP synthase. Pyruvate & Malate (PM), ADP (D), Glutamate (G), Succinate (S), Rotenone (Rot), Oligomycin (Omy), Uncoupler (U), Antimycin (Ama). B Representative respirometric traces from spinal cord tissue using a substrate-uncoupler-inhibition protocol to measure oxygen consumption of the Naïve group (Gray line), the SNC group (Red line), the SNC^(HBOT) group (Blue line) after 12 days of sciatic nerve injury. Blue-Thin line represents the oxygen concentration of the chamber (left y axis) and the oxygen flux per mass (right y axis). C Basal mitochondrial respiration in normal state (without stimulators or inhibitors). D Mitochondrial respiration in the non-phosphorylation resting states-LEAK. E Maximal mitochondrial respiration (CI + CII). F Mitochondrial respiration in the OXPHOS states coupled to phosphorylation of ADP to ATP in the presence of saturating ADP (CI, CII, CI + CII). G Mitochondrial respiration in the Electron transfer (ET) capacity state. (n = 7–10). One-way Anova-Followed with Tukey’s test. Mean ± SEM. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001