Fig. 4

HBOT reduces neuronal apoptosis and neuromodulation in the spinal cord after nerve crush. A The transcription level of BAX and Bcl-2 in the spinal cord after 12 days of nerve crush. B The ratio of Bax/Bcl-2 in the spinal cord after nerve crush. C The transcription level of TRPV1 and Caspase-3 in the spinal cord after 12 days of SNC. D The number of cells that were IR-positive for cleaved Caspase-3 in the spinal cord. E Representative images of cleaved Caspase-3 in the spinal cord of the naïve, SNC and SNC^(HBOT) group after 12 days of nerve injury. scale bar: 100 µm. RT-PCR (4–7), IHC (n = 4–6 animals). One-way Anova-Followed with Tukey’s test. Mean ± SEM. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001