Fig. 3

The effect of HBOT on neuronal apoptosis and neuromodulation in the DRG after nerve crush. A The transcription level of BAX and Bcl-2 in the sciatic nerve/DRG after 12 days of nerve crush. B The ratio of Bax/Bcl-2 after 12 days of nerve crush. C The transcription level of TRPV1 and Caspase-3 in the sciatic nerve/DRG after 12 days of nerve crush. D Double immunofluorescence of TRPV1 (Green) and cleaved Caspase-3 (Red), Co-localization of TRPV1 and cleaved Caspase-3 (Orange) in the DRG after 12 days of nerve crush. scale bar: 100 µm. E The number of cells that were IR-positive for cleaved Caspase-3. F The number of cells that were IR-positive for TRPV1. G The percentage of cells that were IR-positive for both TRPV1 and cleaved Caspase-3 in the DRG. RT-PCR (4–7), IHC (n = 4–6 animals). One-way Anova-Followed with Tukey’s test. Mean ± SEM. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001