Fig. 4

AKR1B10-mediated PEM resistance is dependent on the enhanced effects on anaerobic glycolysis. A, D Indicated cells were treated with different doses of PEM with or without 2-DG (2.5 mM) for 72 h and CCK-8 assays were performed to determine their viability. B, E Results of clonogenic assays showing cell survival of the indicated cells after treatment with PEM (20 nM) with or without 2-DG (2.5 mM). Pixel density quantification of clonogenic assays is shown as histogram. C, F Flow cytometry analysis of the indicated cells after treatment with PEM (20 nM) with or without 2-DG (2.5 mM) for 24 h. (n = 3, *p < 0.05, **p < 0.01, ****p < 0.0001, PC9-NC, PC9 transfected with negative control plasmid; PC9-OE, PC9 transfected with AKR1B10 plasmid. shNC, PC9-BrM3 cells transfected with negative control shRNA; shAKR1B10, PC9-BrM3 cells transfected with AKR1B10-targeted shRNA vector 1)