Fig. 7

Studies on JorA-interaction proteins. A Schematic diagram of fishing JorA-interaction proteins. B The protocol for biotin-JorA pulling-down experiment. C Comparison of the anticancer activity between biotin-JorA and native JorA. Data was collected in three independent repeats. Error bars represent standard deviation. P-values were derived from Two-tailed Student’s t-test; ***p < 0.001; ns means no significant. D The Venn diagram of three mode samples mass spectrum identification results is shown in the figure. Simulation represents computeral simulation through Pharm Mapper platform (http://lilab-ecust.cn/pharmmapper/), pulldown (in vivo) and pulldown (in vitro) has been explained in Materials and Methods section. E Pulldown followed by western blotting to test the JorA-interaction proteins FASN and TOP1. F The differential expression of FASN in bladder tumor tissues and normal bladder tissues. Data are obtained from GEPIA. G The relationship between the FASN expression level and the survival rate of bladder cancer patients. H The docking structure of molecular interaction between JorA and FASN. JorA and FASN were colored cyan and green respectively. I The docking structure of molecular interaction between JorA and TOP1. JorA and TOP1 were colored cyan and lightpink respectively