Fig. 4

Phenotype evidence of JorA repressing proliferation, migration and inducing apoptosis. A EdU staining to investigate JorA inhibiting DNA replication in bladder cancer T24 cells. EdU positive stained cells were shown as red fluorescence; Cells nuclei were shown as blue fluorescence. B Quantitative analysis chart of EdU experiment results. The statistical difference was analyzed by one-way ANOVA followed by Tukey post-hoc analysis, *p < 0.05. C The scratch test was employed to observe cell migration. D Quantitative analysis of cell migration experiment results. The relative migration rate of control group (without JorA) of 48 h was 100%. The statistical difference was analyzed by one-way ANOVA followed by Tukey post-hoc analysis, *p < 0.05. E To test apoptosis, Annexin V-FITC/HO33342 staining was performed. Annexin V-FITC positive cells were stained as green fluorescence, and the cells nuclei were stained as blue fluorescence. F Quantitative analysis of JorA-induced apoptosis. The statistical difference was analyzed by one-way ANOVA followed by Tukey post-hoc analysis, *p < 0.05