Fig. 3

Proteomics analysis reveals JorA modulates proliferation and apoptosis related molecules in MIBC T24 cells. A The identification and quantitative results statistics of spectrum, peptides and proteins. B The number of significantly upregulated and downregulated proteins by JorA. C Pie chart of proteomics organelle localization analysis. D Volcanic map of JorA induced significantly changed proteins. Gray dots indicate genes with no significant difference, red dots indicate upregulated genes with significant differences, and blue dots indicate downregulated genes with significant differences. E Top 20 pathways of KEGG enrichment analysis result. F JorA modulated key molecules (such as KEAP1, Bid, LAMB3, EGFR, Bcl-XL, Bcl-2, and PAX8) in “pathways in cancer”. Red boxes indicate upregulated molecules, and green boxes indicate downregulated molecules. G Heat map of JorA-modulated molecules in "paths in cancer". CTL refers to the control group; JAT refers to the JorA treatment group. H Western blotting to test the protein level of Bcl-2 and EGFR. I The relationship between EGFR expression level and survival rate of bladder cancer patients. J The expression levels of Ki67 and EGFR in xenografted bladder tumor were detected by IF staining