Fig. 1

JorA specifically inhibited MIBC cells in vitro. A Schematic of JorA synthesis steps. B The 3D structure of JorA. C SW780, T24, UM-UC-3, SW480, U251 and H293T cells were incubated with 0–0.4 μM JorA for 48 h. Cell activity was tested using a CCK-8 kit. D SW780, T24, UM-UC-3, SW480, U251 and H293T cells were incubated with 0.1 μM of JorA for 48 h. Data was collected in three independent repeats. P-values were derived from Two-tailed Student’s t-test, *p < 0.05. E T24 cells were incubated with 0–0.2 μM of JorA. The IC50 is 0.054 μM. F UM-UC-3 cells were incubated with 0–0.2 μM of JorA. The IC50 is 0.084 μM. G T24 cells were treated by 0–0.4 μM of JorA for 48 h. Cellular morphology changes were examined under a microscope. Orange arrows indicate T24 cells with morphological alteration, red arrows indicate cells with decreased extensibility, green arrows indicate cells with membrane perforation, blue arrow indicated dead cells. H T24 cells were incubated with 0–0.2 μM of JorA for 14 days. The cells was fixed by 4% PFA and stained with crystal violet. I Quantitative calculation of clonogenic capacity at different concentrations of JorA. The statistical difference was analyzed by one-way ANOVA followed by Tukey post-hoc analysis, *p < 0.05