Fig. 4

Effects of NAG treatment on the expression of IL-1β, IL-18, IL-6, TNF-α, p-p65, NLRP3, GSDMD, pro-caspase-1 and caspase-1 in the ventral horn. The expression levels of NLRP3 (A, B), GSDMD (C, D), caspase-1/pro-caspase-1 (E, F), TNF-α (G, H), IL-1β (I, J), IL-18 (K, L), IL-6 (M, N) and p-p65 (O, P) in the ventral horn of the spinal cord were determined using Western blotting. The data shown are the mean ± SD in each group; ***P < 0.001, **P < 0.01, *P < 0.05. NS normal saline, NAG acetylglutamine