Fig. 3

Effects of NAG treatment on histological alterations and motoneuronal survival in the spinal cord. A Histological images of Nissl-stained spinal cord sections in the groups. Scale bar represents 20 μm. B The survival rate of motoneurons was estimated as the percentage of the left (intact)/right (injury) motoneurons located in the ventral horn. The expression levels of GAP-43 (C, D), p-Akt1 (E, F) and nNOS (G, H) in the ventral horn of the spinal cord were determined using Western blotting. The data shown are the mean ± SD in each group; ***P < 0.001, **P < 0.01, *P < 0.05. NS normal saline, NAG acetylglutamine