Fig. 5

Screening for potential metabolic biomarkers of intestinal fibrosis in mice. A Top15 metabolites screened by the random forest algorithm after standardized correction according to the prediction accuracy of sample grouping, with red representing high expression and blue representing low expression. B Spearman’s rank correlation between the top15 metabolites and 4 fibrotic indicators, which reflect the degree of intestinal fibrosis in mice. C AUC heatmap of the top15 metabolites (only six metabolites with AUC > 0.7 among the three groups). D Heatmap of the relative abundance of the six metabolites screened by the random forest algorithm and ROC curves. Fibrotic indicators: α-SMA, Collagen I, and Collagen III correspond to the mRNA level of α-SMA, Collagen I, and Collagen III genes, respectively, in colon samples detected by qPCR. Collagen deposition area of colon (%): quantitative results of collagen deposition determined by ImageJ software. n = 5 per group. *: P < 0.05, **: P < 0.01, ***: P < 0.001. KEGG Kyoto Encyclopedia of Genes and Genomes, AUC area under the curve, ROC receiver operating characteristic