Fig. 4

Correlations between lipid metabolism-related genes and lipid metabolites. A KEGG joint-pathway analysis of the DEGs in Pattern1 and the 48 SAMs; the nine metabolic pathways with P < 0.05 are highlighted with their names. B Cluster heatmap of the relative abundances of the 14 lipid metabolites screened from the 48 SAMs. C Spearman’s rank correlation between the 14 metabolites and 4 fibrotic indicators reflecting the degree of intestinal fibrosis in mice. D The top15 hub genes screened by PPI network and CytoHubba plug-in with degree values. E Spearman’s rank correlation between the top15 hub genes and 4 fibrotic indicators. F Spearman’s rank correlation between the top15 hub genes and 14 lipid metabolites. Fibrotic indicators: α-SMA, Collagen I, and Collagen III correspond to the mRNA level of α-SMA, Collagen I, and Collagen III genes, respectively, in colon samples detected by qPCR. Collagen deposition area of colon (%): quantitative results of collagen deposition determined by ImageJ software. n = 5 per group. *: P < 0.05, **: P < 0.01, ***: P < 0.001