Fig. 5

The impacts of COX-2 inhibitor in murine ITP models. Passive ITP murine models were established and treated with or without COX-2 inhibitor celecoxib. (N = 6) A Basal platelet count of mice in COX-2 inhibitor group and control group before the establishment of ITP models. Untreated and treated represent the platelet counts of mice before and after the gavage of COX-2 inhibitor or control solvent for six days respectively. B Platelet count of mice in COX-2 inhibitor group and control group during the modeling procedure. C Percentages of CD41⁺CD61⁺ megakaryocytes in bone marrow cells. Flow cytometry dot plots showed the percentages and gates for CD41⁺CD61⁺ CD41⁺CD61⁺ megakaryocytes. D MFI of CD41 on CD41⁺CD61⁺ megakaryocytes. Histogram plots showed the fluorescence intensity of CD41 in flow cytometry. E, F Percentages of CD62P⁺ cells E and Annexin V⁺ apoptotic cells (F) in CD41⁺CD61⁺ megakaryocytes. Flow cytometry dot plots showed the percentages and gates in CD41⁺CD61⁺ megakaryocytes. G Percentages of polyploid cells in CD41⁺CD61⁺ megakaryocytes. Histogram plots showed the percentages and gates for polyploid cells (N > 4) in CD41⁺CD61⁺ megakaryocytes. H HE staining of bone marrow of ITP mice in COX-2 inhibitor group and control group. Plots inside rectangular boxes are magnified and showed below. Arrows marked mature megakaryocytes. I Immunofluorescence staining of bone marrow of ITP mice in COX-2 inhibitor group and control group. DAPI was used to identify nucleus. Arrows marked mature megakaryocytes. Megakaryocytes inside rectangular boxes are magnified and showed at the lower right corner of plots. * P < 0.05, **P < 0.01, ***P < 0.001