Fig. 4

LIGHT contributes to monocyte migration and macrophage M2 polarization. a Representative images of mouse cardiac tissues from the different groups with CD3, F4/80, MCP1, iNOS, CD163, and CD206 IHC staining (from above to below). b Quantification of CD3⁺, F4/80⁺, MCP1⁺, iNOS⁺, CD163⁺, and CD206⁺ areas in the different groups. c mRNA levels of MCP1, TNF-α, IL-1β, IL-10, TGF-β1, ARG1, and CD163 in the different groups [relative to the LIGHT( −) group]. d Elevation in the serum levels of TNF-α, IL-1β, TGF-β1, IL-10, and IL4 from different groups. e Western blot analysis of MCP1, iNOS, TGF-β1, ARG1, CD163, and CD206, and f relative expression in the cardiac tissue normalized to the control. *p < 0.05, **p < 0.01. MCP1: monocyte chemoattractant protein-1; TNF-α: tumour Necrosis Factor α; IL-1β: interleukin 1β; IL-10: interleukin 10; TGF-β1: transforming growth factor β; ARG1: arginase1