Fig. 8

Delayed LIPUS intervention still reduced brain damage after ICH. A Representative MRI T2-weighted images (T2WIs) at 1 day and 3 days post-ICH. LIPUS treatment performed two hours (P2h) after ICH. The contour of lesion volumes was defined by the contrast provided by T2WIs of the brain. Quantification revealed that lesion volumes were significantly reduced after delayed LIPUS intervention. B Cresyl violet staining showed injury volumes and hemispheric enlargement were significantly decreased after delayed LIPUS treatment (scale bar: 2 mm). C Results of brain water content demonstrated that the ICH + LIPUS group exhibited significantly less brain edema than the ICH group in the ipsilateral basal ganglia area at D3. (Ipsi-CX: ipsilateral cortex, Contra-CX: contralateral cortex, Ipsi-BG: ipsilateral basal ganglia, Contra-BG: contralateral basal ganglia, Cere: cerebellum) D Representative MBP-stained and E FJB-stained sections of a sham, an ICH, and a delayed LIPUS-treated group at 3 days post-ICH. ICH-induced myelin loss and neurodegeneration were significantly ameliorated after delayed LIPUS treatment (scale bar: 50 mm). ∗ , #, denote significant difference from the sham group and ICH group, respectively (#P < 0.05, ##P < 0.01, ***, ###P < 0.001; n = 6–8)