Fig. 4

LIPUS treatment decreased neurological deficits and brain damage after ICH. A LIPUS significantly improved the mNSS on 3 days post-ICH (***P < 0.001 vs D0, #P < 0.05 vs ICH; n = 8). B There were no significant differences in body weight between the ICH and ICH + LIPUS groups in the 3 days after ICH (**P < 0.01, ***P < 0.001 versus D0; n = 8). C We observed a significant reduction in brain water content in the ipsilateral basal ganglion area of the ICH + LIPUS group compared to the ICH group. (Ipsi-CX ipsilateral cortex, Contra-CX contralateral cortex, Ipsi-BG ipsilateral basal ganglia, Contra-BG contralateral basal ganglia, Cere cerebellum; **P < 0.01 vs Contra-CX; ###P < 0.001 vs ICH at Ipsi-BG; n = 8). Results of histological staining analysis demonstrated that mice in the ICH + LIPUS group exhibited significantly (D) lower injury volume and hemispheric enlargement (#P < 0.05, ##P < 0.01 vs ICH; n = 8), E higher myelin basic protein expression (MBP [green], DAPI [blue]; ***P < 0.001 vs sham, ###P < 0.001 vs ICH; n = 8), and F lower FJB-positive cell numbers than the ICH group (***P < 0.001 vs sham; #P < 0.05, ##P < 0.01 vs ICH; n = 8). In addition, G ICH-induced levels of cleaved caspase-3 expression on D3 were reduced after LIPUS treatment (***P < 0.001 vs sham; #P < 0.05, vs ICH; n = 7–8)