Fig. 2

Time-course expressions of microglia and astrocyte were measured after ICH. A Time schedule of the study. Western blot analysis of (B) Iba1, and (C) GFAP protein levels were significantly upregulated at 1 day and/or 3 days post-ICH. Immunofluorescence double labeling for nuclei (blue) and microglia (Iba1, green) or astrocyte (GFAP, green) showed that (D) numbers of Iba1-positive cells activated from 3 h to 3 days, and (E) intensity levels of GFAP induced at 3 days post-ICH. *denotes significant difference from the sham group (*P < 0.05, **P < 0.01, ***P < 0.001; n = 5)