Fig. 6

UCP1 alleviates ROS production in renal fibrosis by regulating the protein stability of SIRT3. A inBio Discover website showing highly correlation between UCP1 and SIRT3. B Photomicrographs displaying immunohistochemical staining against SIRT3 in UUO mice for 3, 7, and 14 days. C Aggregated statistics of integrated optical density (IOD)/area (n = 5). D Western blot results and E corresponding quantification of SIRT3 in HK2 cells exposed to TGF-β1 for 12, 24 and 48 h. F Western blot results and G corresponding quantification of SIRT3 in HK2 cells in different cell groups. H Western blot images and I corresponding quantifications of SIRT3 in different cell groups. J Immunofluorescence images of ROS in HK2 cells in different cell groups. * P < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. Ctrl or Sham