Fig. 5

Upregulation of UCP1 and CL316243 treatment inhibits ROS production in UUO mice and TGF-β1 stimulated TECs. A Immunofluorescence images of ROS in HK2 cells in different cell groups. B Relative MDA content in HK2 cells specified in A. C Representative images and D corresponding quantification of SOD2 and CAT in HK2 cells specified in A. E Immunofluorescence images of ROS in HK2 cells in different cell groups. F Relative MDA content in HK2 cells specified in E. G Representative images and H corresponding quantification of SOD2 and CAT in HK2 cells defined in E. I Representative images and J corresponding quantification of SOD2 and CAT in Sham and UUO mice with control or UCP1-expressing adenovirus injection (n = 5). K Representative images and L corresponding quantification of SOD2 and CAT in Sham and UUO mice with or without CL316243 therapy (n = 5). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. Ctrl or Sham; # vs. TGF-β1 or UUO