Fig. 3

CL316243 treatment significantly alleviates EMT, ECM accumulation and renal interstitial fibrosis in UUO mice. A Specific administration methods of animal models. B Photomicrographs showing immunohistochemical staining against UCP1 in Sham and UUO mice with or without CL316243 treatment. C Aggregated statistics of integrated optical density (IOD)/area (n = 5). D Representative images and E corresponding quantification of UCP1 in animal models specified in B (n = 5). F Representative micrographs of HE and Masson staining of animal models defined in B. G Relative collagen volume fraction of animal models specified in B (n = 5). H Representative images and I corresponding quantification of E-cadherin and α-SMA in animal models defined in B (n = 5). J Immunofluorescence images of Fibronectin in animal models defined in B. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. Sham; # vs. UUO