Fig. 2

Upregulation of UCP1 alleviates EMT, ECM accumulation and renal interstitial fibrosis in UUO mice. A Specific administration methods of animal models. B Representative images and C corresponding quantification of UCP1 in animal models with UCP1-expressing adenovirus or blank control injection (n = 5). D Photomicrographs showing immunohistochemical staining against UCP1 in Sham and UUO mice with UCP1-expressing adenovirus or blank control injection. E Aggregated statistics of integrated optical density (IOD)/area (n = 5). F Representative photomicrographs of HE staining and Masson’s trichrome staining of sham and UUO animal models defined in D. G Relative collagen volume fraction of animal models defined in D (n = 5). H Representative images and I corresponding quantification of E-cadherin and α-SMA in animal models defined in D (n = 5). J Immunofluorescence images of Fibronectin in animal models defined in D. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. Sham; # vs. UUO