Fig. 1

UCP1 is downregulated in TIF patients, UUO mice and TGF-β1 stimulated TECs. A Immunohistochemical images of UCP1 in the kidney cortex from patients with TIF and samples from nephrectomized patients. B Micrograph displaying immunohistochemical staining against UCP1 in UUO mice for 3, 7, and 14 days. C Aggregated statistics of integrated optical density (IOD)/area (n = 5). D Representative images and E corresponding quantification of UCP1 in UUO mice for 3, 7, and 14 days (n = 5). F Representative images and G corresponding quantification of UCP1 in HK2 cells exposed to TGF-β1 for 12, 24 and 48 h. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. Ctrl or Sham