Fig. 6

Telmisartan enabled axon regeneration after spinal cord contusion in mice. A Representative western blot image of the expressions of AGTR1 at 5 days after SCI. B Quantification of the expression of AGTR1 (normalized to levels in the Sham group) in spinal cord tissues in each group (n = 3, ***P < 0.001, one-way ANOVA followed by post hoc Tukey’s test). C Representative immunofluorescence images of serotonergic axons (5-HT, red) staining and DAPI (blue) in the sagittal view of the spinal cord. Bar = 200 µm and 25 µm (the enlarged images of the region of interest in the leftmost panorama). D Representative immunofluorescence images of NF200 (green) staining and DAPI (blue) in the sagittal view of the spinal cord. Bar = 200 µm and 25 µm (the enlarged images of the region of interest in the leftmost panorama). E Quantification of the NF200-positive intensity in the injured center (n = 3, *P < 0.05, ***P < 0.001, one-way ANOVA followed by post hoc Tukey’s test). F Quantification of serotonergic axons at an indicated distance beyond the lesion (n = 3, ***P < 0.001, two-way ANOVA followed by post hoc Tukey’s test, * denominated as the comparison between the SCI group and the telmisartan group)