Fig. 7

Proteomic analysis of cell culture supernatants from the CMV model under suppressive conditions. Monocytes and T cells were isolated from peripheral blood mononuclear cells of donors screened for having CMV-specific T cells. Monocytes were differentiated with GM-CSF/IL-4 to immature dendritic cells (DCs). DCs were infected with LOAd(-), LOAd703 or LOAd732, or stimulated with Poly(I:C)/TNFα (positive control), or left untreated. 24 h later, DCs were pulsed with the CMV peptide pp65 and co-cultured with autologous T cells with and without the addition of TGF-β1 and IL-10. After 11 days of co-culture, cell culture supernatants were harvested and analyzed with Olink Target 96 Immuno-Oncology multiplex assay. The box plots show the median and quartiles and whiskers display the minimum and maximum (n = 6). The data is displayed as a percentage fold change compared to control conditions. In A, markers connected to T-cell activation are shown (A). B displays T and natural killer (NK) cell effector molecules (B). Graphs in C and D show NK cell specific (C) and DC specific markers (D), respectively. E displays chemokines and cytokines (E). Statistical differences between LOAd samples and positive control samples were determined with Kruskal–Wallis test followed by Dunn’s multiple comparison test (*p < 0.05, **p < 0.01, ***p < 0.001)