Fig. 2

Oncolysis in vitro and in vivo. In A, melanoma cells Mel526 and Mel624 were infected with LOAd(-), LOAd703 or LOAd732, or left uninfected. 48 h later, the relative viability of the tumor cells was assessed with CellTiter 96 AQueous One Solution MTS reagent. The bar graphs show the pooled data of three biological repeats and the mean ± SD of technical quadruplicates. Statistical differences between LOAd-infected and uninfected cells were determined with Kruskal–Wallis test followed by Dunn’s multiple comparison test (*p < 0.05, **p < 0.01, ****p < 0.0001) (A). In B, Mel526 or Mel624 cells were injected subcutaneously in female immunodeficient BALB/c nude mice. Treatments with saline control, LOAd(-) or LOAd732 were initiated when tumors were detectable in at least 50% of mice (Mel526: day 7, Mel624: day 17/18) and given intratumorally two times per week for 3 weeks. Tumor growth was monitored and mice with tumors over 1000 mm³ were sacrificed. The graphs show the individual tumor growth curve for each mouse and the pooled data of two experimental repeats (10 mice in total per group) (B)