Fig. 1

Transgene expression in melanoma cell lines. Melanoma cells Mel526 and Mel624 were infected with LOAd(-), LOAd703 or LOAd732, or left uninfected. 48 h later, cell culture supernatants and cells were harvested for transgene expression analysis. Expression of CD40L (A) and 4-1BBL (B) on the cells was determined with flow cytometry and is shown as percentage positive cells, whereas IL-2 concentrations (C) in the culture supernatants were measured with ELISA. The bar graphs show the mean ± SD of three biological repeats. Statistical differences between LOAd-infected and uninfected cells were determined with Kruskal–Wallis test followed by Dunn’s multiple comparison test (*p < 0.05)