Fig. 8

AKT/GSK-3β/β-Catenin pathway regulates the tumor-promotion effect of CD58 in HCC cells. a, b CCK-8 assay (a) and colony formation (b) analysis indicated that LY294002 treatment eliminated CD58 knockdown-mediated suppression of HCC cell growth. c, d SC-79 attenuated HCC cell’s inhibition of migration and invasion induced by CD58 knockdown in SK-Hep-1 (c) and SNU-449 (d) cells. e Spherical formation experiments showed that SC-79 treatment eliminated the inhibition of CD58 silencing on the stemness of SK-Hep-1 and Huh7 cells. f SK-Hep-1 and SNU-449 cells with stable CD58 depletion were incubated with SC-79 for 24 h, followed by western blotting with the corresponding antibodies (n = 3)