Fig. 7

CD58 regulates β-catenin activity by modulating AKT/GSK-3β signaling in HCC cells. a Western blotting analysis of the protein levels of β-Catenin, p-β-catenin (Ser552), GSK-3β, p-GSK-3β(Ser9), AKT, and p-AKT(Ser473) (n = 3). b, c Protein expression of the stable CD58-knockdown SK-Hep-1 (b) and SNU-449 (c) cells transfected with GSK-3β were detected by western blotting with corresponding antibodies (n = 2). d SK-Hep-1 and SNU-449 cells with stable CD58 knockdown were incubated with SC-79 for 24 h, followed by western blotting with the corresponding antibodies (n = 3)