Fig. 6
CD58 activates the Wnt/β-catenin signaling pathway in HCC cells. a The association between CD58 and β-catenin from the TCGA database. b TOP luciferase activity assay revealed the effect of CD58 silencing on TCF/LEF-mediated transcription activity. c Western blotting analysis of the levels of CD58, total β-catenin, active β-catenin (S33/37/T41), Cyclin D1, and c-Myc protein (n = 3). d Immunofluorescence analysis of the localization of active β-catenin. e A subcellular fractionation assay was performed to isolate the nuclear and cytoplasmic fractions and evaluated the expression of active and total β-catenin (n = 2)