Fig. 4

CD58 and sCD58 increase HCC cell migration and invasion. a, b The left panel displays typical pictures of migration (a) and invasion (b) assays for CD58 knockdown and control HCC cells, while the right panel displays the statistical results of Transwell assays. Five randomly chosen single fields of vision were used to count the cells under the microscope at 10 × magnification. c, d ELISA was used to measure the concentration of sCD58 in HCC cell supernatants (c) and serum of HCC patients and healthy individuals (d). e, f Transwell assays were used to assess the migration (e) and invasion (f) capabilities of HCC cells that had been incubated with various concentrations of sCD58. g Western blotting analysis of E-Cadherin and vimentin protein levels in the indicated cells (n = 3)