Fig. 3

CD58 promotes the proliferation of HCC cells in vitro and in vivo. a CD58 protein levels in seven HCC cell lines were measured by western blotting. b Western blotting analysis of CD58 knockdown efficiency. c, d CCK-8 assay (c) and colony formation (d) analysis were conducted to determine the impact of CD58 depletion on the proliferation of SK-Hep-1, SNU-449, and Huh7 cells. e Huh7 cells expressing control (shcon) or CD58 shRNA (shCD58) were used for in vivo tumorigenesis. Tumor volume at indicated time (days) after tumor inoculation. f Tumors images and weight at day 29 after tumor inoculation