Table 2 Primers used for real-time PCR
From: Identification of pyroptosis-related genes and potential drugs in diabetic nephropathy
Gene | Species | Primer sequence (5′ to 3′) |
|---|---|---|
ALB | Mouse | Forward: TGTGTTGCCGATGAGTCTGC |
Reverse: ACGGAGGTTTGGAATGGCAC | ||
C1QB | Mouse | Forward: CTGCCTCTAGGGACCCAGACTTC |
Reverse: ATGGAATCCTGGTGTTCTGTGATGC | ||
CASP1 | Mouse | Forward: GAGAAGAGAGTGCTGAATCAG |
Reverse: CAAGACGTGTACGAGTGGTTG | ||
CASP8 | Mouse | Forward: GGATGTTGGAGGAAGGCAATCTGTC |
Reverse: GGGAGAAATCTGGGCATTGTCTGG | ||
CD44 | Mouse | Forward: CCTGGCACTGGCTCTGATTCTTG |
Reverse: CTGTCTTCCACCGTCCCATTGC | ||
COL3A1 | Mouse | Forward: GGTGTAAAGGGTGAACGTGGTAGTC |
Reverse: TTGCCAGGAGGACCAGGAAGAC | ||
EGF | Mouse | Forward: GCCAGGGATGGAAACCTGTG |
Reverse: ATCCCGTCTCCTTCGTAGCC | ||
FN1 | Mouse | Forward: GAGCAAGCCTGAGCCTGAAG |
Reverse: GGCGCTCATAAGTGTCACCC | ||
GPR183 | Mouse | Forward: TCTGCCTGTCCGTCTGGATTCTG |
Reverse: GTGGTCTTGTCTCCCTCCTCCTTAG | ||
HPGDS | Mouse | Forward: CAATCCACCAGAGCCTCGCAATAG |
Reverse: CATGAAGTCATCCAGCGTGTCCAC | ||
IL18 | Mouse | Forward: CAAAGAAAGCCGCCTCAAACCTTC |
Reverse: TTGACGCAAGAGTCTTCTGACATGG | ||
NEGR1 | Mouse | Forward: AGCCTTGCGTTGACACTATCTTCTG |
Reverse: AGCCATCAGCACTCCCAGAGAC | ||
SPON1 | Mouse | Forward: GGACAGAAGGGCAAGGACAGTAATG |
Reverse: GGGTAAGGCTCGGAGGTCAGTC | ||
TP53 | Mouse | Forward: GGACCCTGGCACCTACAATGAAATC |
Reverse: CCCTGGAGGATATGGACCCTATGAG | ||
β-actin | Mouse | Forward: AGAGGGAAATCGTGCGTGAC |
Reverse: CAATAGTGATGACCTGGCCGT |