Fig. 1

GAA affects mitochondrial function in ovarian cancer cells. A High-throughput screening of OXPHOS inhibitors based on natural compounds in ovarian cancer cells. GAA was screened as a candidate OXPHOS inhibitor. B, C Cell inhibition rates determined using MTT following treatment with natural compounds (n=852). D Relative fluorescence intensity in A2780 cells treated with candidate natural compounds. GAA vs NC, n=3, **P<0.01. E Chemical constituents of gossypol acetic acid (GAA). F Measurement of mitochondrial membrane potential via Mito Tracker staining in A2780 cells treated with GAA