Fig. 2

ESRRG suppresses proliferation of ESCC cells in vitro and in vivo. A, B Cell viability of ESCC cells with ESRRG overexpression or knockdown compared to control vector group were analyzed using was determined by the CCK-8 assay (n = 3). C Effects of ESCC cells with ESRRG overexpression and knockdown compared to control vector group on colony formation (n = 4). D The representative pictures of two independent ESCC organoids transfected with ESRRG overexpression vectors or control lentivirus for 2 weeks were shown and quantified via organoid diameters (n = 10) E, F Effects of ESCC cells with ESRRG overexpression and knockdown on EDU assay (n = 3). G Image of tumors isolated from nude mice with tumor xenografts derived from the indicated groups. Effects of ESRRG overexpression or knockdown on tumorigenicity in nude mice. H Tumour volumes were monitored at indicated time points. I The weight of tumours was measured at time of sacrificed (n = 5). Values are presented as mean ± SD. *P < 0.05 or **P < 0.01 indicates significant differences from the vehicle group as assessed by a one-way ANOVA with a post hoc Dunnett’s test